Last data update: May 06, 2024. (Total: 46732 publications since 2009)
Records 1-3 (of 3 Records) |
Query Trace: Rouphael NG[original query] |
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Streptococcus mitis Expressing Pneumococcal Serotype 1 Capsule.
Lessa FC , Milucky J , Rouphael NG , Bennett NM , Talbot HK , Harrison LH , Farley MM , Walston J , Pimenta F , Gertz RE , Rajam G , Carvalho MDG , Beall B , Whitney CG . Sci Rep 2018 8 (1) 17959 Streptococcus pneumoniae's polysaccharide capsule is an important virulence factor; vaccine-induced immunity to specific capsular polysaccharide effectively prevents disease. Serotype 1 S. pneumoniae is rarely found in healthy persons, but is highly invasive and a common cause of meningitis outbreaks and invasive disease outside of the United States. Here we show that genes for polysaccharide capsule similar to those expressed by pneumococci were commonly detected by polymerase chain reaction among upper respiratory tract samples from older US adults not carrying pneumococci. Serotype 1-specific genes were predominantly detected. In five oropharyngeal samples tested, serotype 1 gene belonging to S. mitis expressed capsules immunologically indistinct from pneumococcal capsules. Whole genome sequencing revealed three distinct S. mitis clones, each representing a cps1 operon highly similar to the pneumococcal cps1 reference operon. These findings raise important questions about the contribution of commensal streptococci to natural immunity against pneumococci, a leading cause of mortality worldwide. |
Diagnostic importance of hyphae on heart valve tissue in Histoplasma endocarditis and treatment with isavuconazole
Wiley Z , Woodworth MH , Jacob JT , Lockhart SR , Rouphael NG , Gullett JC , Guarner J , Workowski K . Open Forum Infect Dis 2017 4 (4) ofx241 A patient who never resided in an endemic area for dimorphic fungi was diagnosed with Histoplasma capsulatum endocarditis. His diagnosis was suggested by yeast and hyphae on cardiac valve tissue pathology. Isavuconazole was an optimal therapeutic option due to renal dysfunction and anticoagulation with warfarin for mechanical valve replacement. |
Evaluation of serum bactericidal antibody assays for Haemophilus influenzae serotype a
Rouphael NG , Satola S , Farley MM , Rudolph K , Schmidt DS , Gomez-de-Leon P , Robbins JB , Schneerson R , Carlone GM , Romero-Steiner S . Clin Vaccine Immunol 2010 18 (2) 243-7 Haemophilus influenzae type a (Hia) is an important pathogen for some American Indian, Alaskan natives and Northern Canada aboriginal populations. Assays to measure serum bactericidal activity (SBA) to Hia have not been developed or validated. Here we describe two methods for the measurement of SBA: SBA with a viability end-point (CFU counts) and SBA with a fluorometric end-point using alamarBlue as metabolic indicator. Both SBA assays measure Hia-specific functional antibody and correlate with anti-Hia IgG ELISA concentration of naturally-acquired antibodies. |
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